Rare Ellagic Acid Sulphate Derivatives from the Rhizome of Geum rivale L.—Structure, Cytotoxicity, and Validated HPLC-PDA Assay
نویسندگان
چکیده
Two rare sulphate ellagic acid derivatives were isolated from the rhizome of Geum rivale L. in three simple steps. Their structures were identified by comprehensive NMR studies (1H NMR, 13C NMR, 1H-1H COSY, HMBC, HSQC) as 3,3′-dimethoxy-4-sulphoxyellagic acid potassium salt (1) and 3,3′,4′-trimethoxy-4-sulphoxyellagic acid potassium salt (2). Subsequently, a new precise (RSD < 2.6%), accurate (recoveries in the range of 96.5–98.7%), and sensitive (LODs in the range of 0.15–0.16 μg/mL) HPLC-PDA procedure was developed for the simultaneous quantification of compounds 1 and 2 in plant material. The rhizome of G. rivale proved to be a good source of both compounds, with the content of 2.94 ± 0.03 and 5.45 ± 0.03 mg/g dw respectively, whereas at most, trace amounts were detected in related plant materials (aerial parts of G. rivale, rhizome and aerial parts of G. urbanum). The cytotoxicity of isolated compounds tested on human leukaemia (promyelocytic HL-60 and lymphoblastic NALM-6) and melanoma (WM 115) cell lines with IC50 values in the range of 306.4–473.8 μM was demonstrated to be lower than that of ellagic acid (IC50 = 62.3–300.6 μM).
منابع مشابه
Investigation into biologically active constituents of Geum rivale L.
Aerial and underground parts of Geum rivale (Rosaceae) were investigated. Tiliroside, gallic acid, ellagic acid and a sterol fraction were isolated from aerial parts of the plant. The sterol fraction was analyzed using GC-MS. Eleven phenolic acids were identified in aerial parts of the plant, and eight in underground parts, by means of RP-HPLC analysis. The quantitative determination of phenoli...
متن کاملAntioxidant Activity of Geum Rivale
Two species of the Rosaceae genus Geum, comprising high amounts of tannins and phenolic acids, were investigated in terms of their antioxidant activity. Antioxidant potential of different extracts from aerial and underground parts of Geum rivale and G. urbanum was studied using various in vitro methods (FC, DPPH, FRAP and linoleic acid peroxidation test). The hydromethanolic extract from the ro...
متن کاملFatty acid synthase inhibitors from Geum japonicum Thunb. var. chinense.
Bioassay-guided fractionation of the MeOH extract of Geum japonicum Thunb. var. chinense using the fatty acid synthase inhibition assay led to the isolation of a new dimeric ellagitannin, gemin G (1), together with six known compounds, gemin A (2), casuarinin (3), pedunculagin (4), potentillin (5) , tellimagrandin II (6), and ellagic acid (7). Their structures were determined on the basis of sp...
متن کاملDevelopment and Validation of a Reversed-phase HPLC Method for Assay of the Decapeptide Cetrorelix Acetate in Bulk and Pharmaceutical Dosage Forms
A gradient reversed-phase high performance liquid chromatography (HPLC) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (GnRH) antagonistic activity used in infertility treatment. The HPLC method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ICH guidelines. The chromatograph...
متن کاملDevelopment and Validation of a Reversed-phase HPLC Method for Assay of the Decapeptide Cetrorelix Acetate in Bulk and Pharmaceutical Dosage Forms
A gradient reversed-phase high performance liquid chromatography (HPLC) method was developed for the assay of cetrorelix acetate, a synthetic decapeptide with gonadotropin-releasing hormone (GnRH) antagonistic activity used in infertility treatment. The HPLC method, which is used to determine cetrorelix in bulk and pharmaceutical dosage forms, was validated per ICH guidelines. The chromatograph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017